Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbohydrate content, low-stress condition, and high chlorophyll content, suggesting that plastic as a culture vessel provides a good impact for culturing PLBs and has low cost. Different designs of the plant tissue culture vessel, such as size, material, and shape, may alter its microenvironment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vessels of different sizes. PLBs were cultured in half-strength Murashige and Skoog (MS) medium with the same initial weight of 0.5 g in 10 replicates. The growth index of the PLBs was calculated after 11 weeks to study their growth in every vessel; additionally, biochemical analysis was performed to determine carbohydrate content, proline concentration, and photosynthesis pigments in the PLBs. Scanning electron microscopy (SEM) was performed to study stomata development on PLBs in each vessel, and histological analyses were conducted to study the cell structure. Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbohydrate content, low-stress condition, and high chlorophyll content. SEM confirmed that the presence of trichome and rhizoid in PLBs cultured in the 470 ml plastic vessel. Histological analysis showed the formation of the shoot on the PLBs and the presence of starch granules. Thus, the use of plastic as a culture vessel provides a good impact for culturing PLBs and has low cost.
Self-supervised classification of subcellular morphometric
PDF) Impact of culture vessels on micro-morphological features of
Self-supervised classification of subcellular morphometric phenotypes reveals extracellular matrix-specific morphological responses
Frontiers Patient-derived models facilitate precision medicine
IJPB, Free Full-Text
IJMS, Free Full-Text
Human blood vessel organoids reveal a critical role for CTGF in
An analysis modality for vascular structures combining tissue
Endothelial inflammation and neutrophil transmigration are
Organotypic stromal cells impact endothelial cell transcriptome in
Frontiers Microfluidic and Organ-on-a-Chip Approaches to Investigate Cellular and Microenvironmental Contributions to Cardiovascular Function and Pathology
Frontiers 3D-Cultured Vascular-Like Networks Enable Validation of Vascular Disruption Properties of Drugs In Vitro
PDF) Impact of culture vessels on micro-morphological features of