Methods of integrating target genes into the B. subtilis genome: (A)

Methods of integrating target genes into the B. subtilis genome: (A)

Frontiers Highly Efficient Genome Engineering in Bacillus anthracis and Bacillus cereus Using the CRISPR/Cas9 System

CRISPR-Cas9 editing with an erm gRNA. Plasmid pJOE8999 (7.8 kb; [11])

Molecules, Free Full-Text

Schematic overview of the “All-In-One” genome integration method. (A)

Bacillus subtilis genome vector-based complete manipulation and reconstruction of genomic DNA for mouse transgenesis, BMC Genomics

Guoyan ZHANG, Master, Jiangsu University, Zhenjiang

IJMS, Free Full-Text

Integration of a heterologous gene in the B. subtilis chromosome

A simplified method for CRISPR-Cas9 engineering of Bacillus subtilis

Barriers to simultaneous multilocus integration in Bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the CRISPR-Cas9 system

Guoyan ZHANG, Master, Jiangsu University, Zhenjiang

Construction and application of an efficient dual-base editing

Bacillus integrative plasmid system combining a synthetic gene circuit for efficient genetic modifications of undomesticated Bacillus strains, Microbial Cell Factories