Methods of integrating target genes into the B. subtilis genome: (A)
Frontiers Highly Efficient Genome Engineering in Bacillus anthracis and Bacillus cereus Using the CRISPR/Cas9 System
CRISPR-Cas9 editing with an erm gRNA. Plasmid pJOE8999 (7.8 kb; [11])
Molecules, Free Full-Text
Schematic overview of the “All-In-One” genome integration method. (A)
Bacillus subtilis genome vector-based complete manipulation and reconstruction of genomic DNA for mouse transgenesis, BMC Genomics
Guoyan ZHANG, Master, Jiangsu University, Zhenjiang
IJMS, Free Full-Text
Integration of a heterologous gene in the B. subtilis chromosome
A simplified method for CRISPR-Cas9 engineering of Bacillus subtilis
Barriers to simultaneous multilocus integration in Bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the CRISPR-Cas9 system
Guoyan ZHANG, Master, Jiangsu University, Zhenjiang
Construction and application of an efficient dual-base editing
Bacillus integrative plasmid system combining a synthetic gene circuit for efficient genetic modifications of undomesticated Bacillus strains, Microbial Cell Factories